Overexpression of Human DNA Topoisomerase IIα by Fusion to Enhanced Green Fluorescent Protein
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Overexpression of Human DNA Topoisomerase II α by Fusion to Enhanced Green Fluorescent Protein
DNA topoisomerase (topo) IIα is a major target for many anticancer agents. However, progress towards understanding how these agents interact with this enzyme in human cells and how resistance to these agents arises is greatly impeded by difficulties in expressing this gene. Here, we report on achieving a high level of expression of a full-length human topo IIα gene in human cells. We started wi...
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1.Ausubel, F.M., R. Brent, R.E. Kinston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene/Wiley Interscience, New York. 2.Chandler, C.S. and F.J. Ballard. 1986. Multiple biotin-containing proteins in 3T3-L1 cells. Biochem. J. 237:123-130. 3.Dignam, J., R. Lebovitz and R. Roeder. 1983. Accurate transcription initiation by RNA polymerase II ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98256cr04